Research

Our Recent Work

Our research focuses on understanding the impact of heat stress on wheat reproductive and root development through advanced cytological, anatomical and biochemical analysis studies.

✅ Phenotyping :

We analyze plant characteristics in field conditions, assessing traits like plant height, leaf structure, yield, and stress responses.

✅ Root Phenotyping:

We are also performing root sectioning to examine xylem and phloem development under heat stress. By counting xylem and phloem numbers, we aim to understand how heat stress impacts root vascular architecture, which is essential for water and nutrient transport in wheat.

✅ Physiology :

Chlorophyll measurement provides insights into plant health and photosynthetic efficiency. We use SPAD meters and spectrophotometric analysis to determine chlorophyll levels.

✅ Biochemical Analysis:

Our biochemical research focuses on understanding heat stress tolerance mechanisms in wheat at the molecular level. One key aspect of our study is analyzing proline accumulation, an important osmoprotectant that helps plants cope with heat-induced stress. By measuring proline levels, we aim to assess the plant’s stress response, cellular stability, and adaptive mechanisms under high-temperature conditions.

✅ Cytology :

We are currently testing pollen viability and quantifying pollen number to assess the effect of heat stress on wheat fertility. These observations help determine how high temperatures influence pollen production and viability, which are critical for successful fertilization and grain formation.

                 

Pollen viability is assessed through staining techniques and microscopy to determine reproductive efficiency and fertility in different plant genotypes. Using a Nikon 50iX microscope, we are analyzing the stigma-anther interaction at three different phases:

• Optimum phase
• Late phase
• Very late phase

These observations, conducted under field conditions, help us understand how heat stress affects pollen reception, fertilization efficiency, and overall reproductive success.

✅ Anatomy: 

Structural analysis of plant tissues, including leaf, stem, and root anatomy, is conducted to understand adaptations, vascular development, and responses to environmental stresses.

Our research aims to enhance plant productivity, stress resilience, and overall sustainability in agricultural systems. Stay connected for updates on our findings!